Isolation of Inactive and G Protein-resistant Adenylyl Cyclase Mutants Using Random Mutagenesis
نویسندگان
چکیده
منابع مشابه
Isolation of inactive and G protein-resistant adenylyl cyclase mutants using random mutagenesis.
We used random mutagenesis and phenotypic rescue of adenylyl cyclase-null Dictyostelium cells to isolate loss-of-function mutations in the enzyme. Mutants were (i) catalytically inactive or (ii) resistant to chemoattractant receptor and guanosine 5'-3-O-(thio)triphosphate stimulation. Both classes of mutants harbored substitutions within the cytoplasmic C1a domain. Mutations that inactivated th...
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Whereas acute stimulation of G i/o-coupled receptors inhibits the activity of adenylyl cyclase, a delayed consequence of persistent activation of the receptors is heterologous sensitization, an enhanced responsiveness of adenylyl cyclase to activators such as forskolin or agonists of G s-coupled receptors. G s-insensitive mutants of adenylyl cyclase type V were used to test the hypothesis that ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1995
ISSN: 0021-9258
DOI: 10.1074/jbc.270.39.22693